Objective: To analyze the content of heavy metals and harmful elements in Holotrichia diomphalia and provide the scientific foundation for the quality control and standard establishment of H. diomphalia. Method: The content of Pb

Cd

Cu

As

Hg constituents was determined by ICP/MS after microwave-assisted digestion and atom absorption spectrophotometry. The Chinese pharmacopoeia(Volume 1) of 2010 edition was used as the standard to evaluate the pollution condition of Pb

Cd

Cu

As

Hg in H. diomphalia. Result: The content of Pb

Cd

Cu

As

Hg constituents determined by ICP/MS had not obvious difference with the ones determined by atomabsorption spectrophotometry. The content of Pb

Cd

Cu

As and Hg determined by ICP/MS was 3.912

0.163

17.386

1.983 and 0.260 mg· kg-1 respectively. The content of Pb

Cd

Cu

As and Hg determined by atom absorption spectrophotometry was 3.777

0.155

18.027

2.044 and 0.252 mg· kg-1 respectively. Conclusion: The concentrations of Cd in the samples from five different areas of H. diomphalia were lower than the limits of Chinese pharmacopoeia

but the concentrations of Pb

Cu

As

Hg in the some samples from different areas were more than the limit standard. The result is helpful to develop the quality standards of H. diomphalia four harmful elements.