Objective: To study the effect of Yishen Hugu decoction on the changes of the blood and urine biochemical levels

the bone structure

bone biomechanical properties in the rat disused osteoporosis. Method: Seventy SD rats were randomly divided into 7 groups:normal group

sham operation group

model group

high dose

medium dose and low dose of Yishen Hugu decoction group (52

26

16.25 g·kg-1)and positive control group (Drynaria fortunei 0.25 g· kg-1) with 10 rats in each group.The rats in the model group

high

medium

low dose group of Yishen Hugu decoction and positive control group were performed sciatic neurotomy.Normal group was given regular food and water other groups were gavaged Yishen Hugu decoction respectively

and the rats in positive control group were gavaged Drynaria fortunei. The blood and urine biochemical levels were measured

the femora biomechanical test and the tibia were decalcified and then stained with HE and Masson stain. Result: The differences of alkaline phosphatase(ALP)

osteocalcin(BGP)

tartrate-resistant acid phosphatase(TRACP)

hydroxyproline (HYP)/creatinine(Cr)

bone mineral density(BMD) and the numbers of nodes and free ends in model group and sham operation group were significant statically (P<0.01). After administration of Yishen Hugu decoction

those indexes in high

medium

low dose group of Yishen Hugu decoction were obviously higher or lower than that in model group (P<0.01). Conclusion: The disused osteoporosis can be prevented effectively by the Yishen Hugu decoction.