木兰脂素对离体大鼠胸主动脉环血管舒张机制和大鼠肾细胞毒性作用

张洪平; 田戈; 杨涛; 陈良; 姜敏

doi:10.11653/syfj2013230209

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木兰脂素对离体大鼠胸主动脉环血管舒张机制和大鼠肾细胞毒性作用

更新时间：2024-01-04

- 木兰脂素对离体大鼠胸主动脉环血管舒张机制和大鼠肾细胞毒性作用
- Vasodilatative Mechanism of Magnolin in Isolated Rat Thoracic Aorta and its Toxicity in Rat Renal Cells NRK
- 中国实验方剂学杂志 2013年19卷第23期页码：209-212
- 作者机构：
- 作者简介：
- 基金信息：
  
  新疆医科大学博士(后)科研启动基金项目(2012102-5);新疆医科大学自治区级重点学科项目(XYDXK50780338)
- DOI：10.11653/syfj2013230209
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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张洪平, 田戈, 杨涛, 等. 木兰脂素对离体大鼠胸主动脉环血管舒张机制和大鼠肾细胞毒性作用[J]. 中国实验方剂学杂志, 2013,19(23):209-212.

ZHANG Hong-ping, TIAN Ge, YANG Tao, et al. Vasodilatative Mechanism of Magnolin in Isolated Rat Thoracic Aorta and its Toxicity in Rat Renal Cells NRK[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 209-212.
张洪平, 田戈, 杨涛, 等. 木兰脂素对离体大鼠胸主动脉环血管舒张机制和大鼠肾细胞毒性作用[J]. 中国实验方剂学杂志, 2013,19(23):209-212. DOI： 10.11653/syfj2013230209.

ZHANG Hong-ping, TIAN Ge, YANG Tao, et al. Vasodilatative Mechanism of Magnolin in Isolated Rat Thoracic Aorta and its Toxicity in Rat Renal Cells NRK[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(23): 209-212. DOI： 10.11653/syfj2013230209.

摘要

目的: 研究木兰脂素（magnolin，Mag）的舒张血管作用、作用机制和细胞毒性。方法: 应用离体血管环技术观察Mag对大鼠胸主动脉环张力的作用，记录去氧肾上腺素（phenylephrine，PE，1 μmol·L-1）预收缩的离体大鼠胸主动脉环张力变化。采用一氧化氮合酶（eNOS）抑制剂L-硝基精氨酸甲酯（L-NAME，100 μmol·L-1）预处理后观察Mag对血管环张力改变的影响，并用MTT方法观察对大鼠肾细胞（NRK）增殖的影响。结果: 木兰脂素（0.1~1 000 μmol·L-1）对PE预收缩的血管环具有浓度依赖的舒张作用，该舒张作用为内皮依赖性。50%最大效应浓度（Half maximal effective concentration，EC50）为24.43 μmol·L-1。内皮型一氧化氮合酶（endothelial nitric oxide synthesis，eNOS）抑制剂-NG-硝基-L-精氨酸甲酯（NG-Nitro-L-arginine methyl ester hydrochloride，L-NAME，100 μmol·L-1）预处理可以抑制Mag对具内皮血管的舒张效应。木兰脂素（0.1~1 000 μmol·L-1）处理NRK的相对增殖率在84.17%和134.60%。结论: 木兰脂素具有内皮依赖性舒张血管作用，其机制可能与内皮型一氧化氮合成酶激活有关，且没有肾细胞毒性。

Abstract

Objective: To characterize vasodilatative effect of magnolin (Mag) on isolated rat thoracic aorta

elucidate its possible action mechanism

and its toxicity in rat renal cells NRK in vitro. Method: The thoracic aorta was isolated from male Sprague-Dawley (SD) rats and the isometric tension of aortic rings induced by phenylephrine (PE

1 μmol· L-1) were measured. To investigate the vasodilation effect of Mag on it were observed in the rings with endothelium intact or endothelium denuded. The rat renal cells NRK were cultured in vitro and MTT assay. Result: Mag (0.1-1 000 μmol· L-1) produced concentration-dependent response

relaxations in PE-contracted aortic rings with endothelium. The half maximal effective concentration (EC50) of Mag was 24.43 μmol· L-1. The vasodilatative effect by Mag was not statistically inhibited by 100 μmol· L-1 (L-NAME) in the preparations with endothelium. The relative growth rates (RGR) of Mag (0.1-1 000 μmol· L-1) were between 84.17% and 134.60%. Conclusion: Magnolin causes relaxation of aortic ring through endothelium-dependent pathway. The mechanisms might be involved in eNOS. Magnolin is safe to rat renal cells NRK with concentration between 0.1 μmol· L-1and 1 000 μmol· L-1.

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