Objective: To explore the method of establishing the animal model of coronary heart disease due to heart and spleen Yang deficiency syndrome and observe the effects of Fuzi Lizhong decoction interference. Method: Hundred Wistar rats were randomly divided into control group

model group

Fuzi Lizhong decoction low-dose group

middle-dose group and high-dose group 5 groups with 20 rats in each group. The model group was applied by incising brown adipose tissue(BAT) then with high fatty diet and cold stimulation every other day for 21 days. From the 22nd day

the model group was given subcutaneous injection with pitutrin

once a day for 7 days and from the 29th day

low-dose group

middle-dose group and high-dose group were ig respectively with Fuzi Lizhong decoction (10

20

40 g·kg-1) based on model group.Once a day for 7 days. To observe the changes of the cardiac function

creatine kinase(CK)

cholinesterase(CHE)

triglycerides(TG)

total cholesterol(TC)

rectal temperature and weight. Result: The model rats were familiar to the pathologic change of CHD hyperlipoidemia

the myocardial ischemia damage and the cardiac insufficiency and low rectal temperature

weight lose

diarrhea of spleen Yang deficiency syndrome. There was significant difference or high significant difference(P<0.05 or P<0.01)

compared with the control group

middle-dose group and high-dose group. Conclusion: It may provide a valuable method for establishment of combined with disease and Zheng animal model by applying multiple methods during the establishment of the animal model of coronary heart disease due to heart and spleen Yang deficiency syndrome. high-dose Fuzi Lizhong decoction could improve the pathologic changes of model rats to some extent.