Objective: The purpose of this study was to investigate the relationship between the extent of blood stasis and hypoxia-related proteins in the advanced gastrointestinal cancer patients. Method: We recruited 46 patients with stage IV advanced gastrointestinal cancer

29 patients after radical resection of digestive tract tumor and 19 health adults as control between April 1

2012 and April 10

2013.Fasting venous blood were extracted in hospital the next day

and ELISA test were performed to examine the expression level of vascular endothelial growth factor(VEGF)

osteopont in(OPN) and carbonic anhydrase IX(CAIX). Result: The patients with advanced cancer

and postoperative cancer group peripheral blood level of VEGF and OPN

CAIX were significantly higher than that of healthy controls. Between advanced cancer group and postoperative cancer group

the former of VEGF and OPN

CAIX respectively was 1.27 times(P>0.05)

1.33 times(P<0.05)

1.48 times(P>0.05) of the latter.In the advanced cancer group

the level of VEGF、CAIX in the severe blood stasis group were significantly higher than the mild group. The former respectively was 1.92 times(P<0.01)

2.70 times(P<0.01) of the latter.The extent of blood stasis and blood stasis symptoms degree were related to the level of VEGF and OPN

CAIX. The heavier degree of blood stasis

the higher level of anoxia related protein. Conclusion: The expression of hypoxia-related proteins increased gradually with the blood stasis degree in the advanced gastrointestinal cancer patients.