贞栀颗粒中女贞子提取效率的影响因素考察及醇提工艺优选

王莹; 曹伟; 贺俊杰; 陈彦; 朱方石

doi:10.11653/syfj2013240032

您当前的位置：

首页 >

文章列表页 >

贞栀颗粒中女贞子提取效率的影响因素考察及醇提工艺优选

更新时间：2024-01-04

- 贞栀颗粒中女贞子提取效率的影响因素考察及醇提工艺优选
- Investigation of Factors Influencing on Extraction Efficiency of Active Constituents in Ligustri Lucidi Fructus and Optimization of Ethanol Extraction Process of Zhenzhi Granules
- 中国实验方剂学杂志 2013年19卷第24期页码：32-35
- 作者机构：
- 作者简介：
- 基金信息：
  
  江苏省名医效方研究开发与评价业务建设项目（BM200903）;江苏省中医药领军人才项目（LJ200913）
- DOI：10.11653/syfj2013240032
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
王莹, 曹伟, 贺俊杰, 等. 贞栀颗粒中女贞子提取效率的影响因素考察及醇提工艺优选[J]. 中国实验方剂学杂志, 2013,19(24):32-35.

WANG Ying, CAO Wei, HE Jun-jie, et al. Investigation of Factors Influencing on Extraction Efficiency of Active Constituents in Ligustri Lucidi Fructus and Optimization of Ethanol Extraction Process of Zhenzhi Granules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 32-35.
王莹, 曹伟, 贺俊杰, 等. 贞栀颗粒中女贞子提取效率的影响因素考察及醇提工艺优选[J]. 中国实验方剂学杂志, 2013,19(24):32-35. DOI： 10.11653/syfj2013240032.

WANG Ying, CAO Wei, HE Jun-jie, et al. Investigation of Factors Influencing on Extraction Efficiency of Active Constituents in Ligustri Lucidi Fructus and Optimization of Ethanol Extraction Process of Zhenzhi Granules[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 32-35. DOI： 10.11653/syfj2013240032.

摘要

目的：优选贞栀颗粒中女贞子和白花蛇舌草的乙醇提取工艺。方法：采用HPLC测定特女贞苷、齐墩果酸和熊果酸含量。通过单因素试验考察影响特女贞苷转移率的因素，以特女贞苷和总三萜酸含量为评价指标，采用正交试验考察乙醇体积分数、乙醇用量、提取次数及提取时间对贞栀颗粒醇提工艺的影响。结果：最佳提取工艺为女贞子粗粉碎后与白花蛇舌草合并，分别加9，7倍量70%乙醇提取2次，每次1.5 h；特女贞苷、总三萜酸提取量分别为19.083，7.163 mg·g-1。结论：优选的提取工艺合理、稳定可行，为贞栀颗粒工业化生产提供参考。

Abstract

Objective: To optimize ethanol extraction technology of Ligustri Lucidi Fructus and Herba Hedyotis from Zhenzhi granules. Method: Contents of oleanolic acid

ursolic acid and specnuezhenide were determined by HPLC.Factors influencing transfer rate of specnuezhenide were investigated by single factor tests

with contents of specnuezhenide and total triterpenoid acids as indexes

orthogonal test was adopted to optimize ethanol extraction technology of Zhenzhi granules by taking the amount and concentration of ethanol

extraction time and times as factors. Result: Optimum extraction process was as following:coarse crushed Ligustri Lucidi Fructus and mixed with Herba Hedyotis

extracting twice with 70% ethanol (9 folds for the first time

7 folds for the second time) for 1.5 h each time;Extraction amount of specnuezhenide and total triterpenoid acids were 19.083 and 7.163 mg·g-1

respectively. Conclusion: This optimized extraction process was reasonable

stable and feasible

which could provide scientific basis for industrial production of Zhenzhi granules.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

女贞子连续超声逆流提取工艺优选

女贞子不同炮制品及不同树龄中齐墩果酸和熊果酸含量研究

基于市售样品质量考察的“猪腰”和“豆豉”女贞子比较

HPLC测定柿子中齐墩果酸与熊果酸的含量