Objective: To optimize opposite dual-frequency ultrasonic extraction technology of total flavonoids from Citri Reticulatae Pericarpium. Method: Hydrophone method was adopted to investigate cavitation effect of single frequency and opposite dual-frequency ultrasonic under different frequency and power.With yield of total flavonoids as index

effects of extraction time

ethanol concentration

ultrasonic electric power and different frequency/power combinations on extraction technology were investigated by single factor tests. Result: Cavitation effect of opposite dual-frequency ultrasonic(20+25) kHz was greater than single frequency ultrasonic(frequency were 20 kHz and 25 kHz

respectively);Under a temperature of (20±0.5)℃

extraction time of 10 min

ethanol concentration of 60%

opposite dual-frequency ultrasonic combination of (20 kHz

25 kHz)

yield of total flavonoids was 4.52%

which was higher than the same extraction condition of single frequency ultrasonic(3.95%

4.03%). Conclusion: Opposite dual-frequency ultrasonic extraction for active ingredients from Chinese herbal medicines was an ideal method

which was closely related to reactor shape

transducer spatial location and other factors.