HPLC梯度洗脱法测定回春酒中淫羊藿苷、宝藿苷Ⅰ、西红花苷-Ⅰ和西红花苷-Ⅱ的含量

冯晓川; 张蕊; 李红燕

doi:10.11653/syfj2013240085

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HPLC梯度洗脱法测定回春酒中淫羊藿苷、宝藿苷Ⅰ、西红花苷-Ⅰ和西红花苷-Ⅱ的含量

更新时间：2024-01-04

- HPLC梯度洗脱法测定回春酒中淫羊藿苷、宝藿苷Ⅰ、西红花苷-Ⅰ和西红花苷-Ⅱ的含量
- Determination of Icariin, Baohuoside Ⅰ, Crocin-Ⅰ and Crocin-Ⅱ in Huichunjiu by HPLC
- 中国实验方剂学杂志 2013年19卷第24期页码：85-89
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- DOI：10.11653/syfj2013240085
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- 纸质出版日期：2013
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冯晓川, 张蕊, 李红燕. HPLC梯度洗脱法测定回春酒中淫羊藿苷、宝藿苷Ⅰ、西红花苷-Ⅰ和西红花苷-Ⅱ的含量[J]. 中国实验方剂学杂志, 2013,19(24):85-89.

FENG Xiao-chuan, ZHANG Rui, LI Hong-yan. Determination of Icariin, Baohuoside Ⅰ, Crocin-Ⅰ and Crocin-Ⅱ in Huichunjiu by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 85-89.
冯晓川, 张蕊, 李红燕. HPLC梯度洗脱法测定回春酒中淫羊藿苷、宝藿苷Ⅰ、西红花苷-Ⅰ和西红花苷-Ⅱ的含量[J]. 中国实验方剂学杂志, 2013,19(24):85-89. DOI： 10.11653/syfj2013240085.

FENG Xiao-chuan, ZHANG Rui, LI Hong-yan. Determination of Icariin, Baohuoside Ⅰ, Crocin-Ⅰ and Crocin-Ⅱ in Huichunjiu by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 85-89. DOI： 10.11653/syfj2013240085.

摘要

目的：采用高效液相色谱法测定回春酒（HCJ）中淫羊藿苷、宝藿苷Ⅰ、西红花苷-Ⅰ和西红花苷-Ⅱ的含量。方法： Hypersil C18色谱柱（4.6 mm×150 mm，5 μm），流速0.8 mL·min-1，流动相A为乙腈，B为1%冰醋酸溶液，检测波长270 nm（淫羊藿苷和宝藿苷Ⅰ）；流动相A为乙腈-甲醇（2:3），流动相B为0.5%磷酸溶液，检测波长440 nm（西红花苷-Ⅰ和西红花苷-Ⅱ）。结果：淫羊藿苷和宝藿苷Ⅰ分别在0.054 6~1.092 μg （r=0.999 7），0.076 2~1.524 μg（r=0.999 1）进样量与峰面积呈良好的线性关系，平均加样回收率分别为97.56%，99.17%，RSD分别为0.94%，0.77%，西红花苷-Ⅰ和西红花苷-Ⅱ分别在0.102 8~2.056 μg（r=0.999 4），0.060 4~1.208 μg（r=0.999 8）进样量与峰面积呈良好的线性关系，平均加样回收率分别为97.54%，96.91%，RSD分别为0.90%，1.27%。结论：该方法测定结果准确、灵敏、重复性好。

Abstract

Objective: To develop an HPLC method for determination of the content of icariin

baohuoside Ⅰ

crocin-Ⅰand crocin-Ⅱ in Huichunjiu (HCJ). Method: An Hypersil C18 column was used as the chromatographic column

the flow rate was 0.8 mL·min-1. The mobile phase A consisted of acetonitrile

the mobile phase B consisted of 1% acetic acid solution

the UV detection wavelength was at 270 nm (for icariin and baohuoside I). The mobile phase A consisted of acetonitrile-methanol (2:3)

the mobile phase B consisted of 0.5% phosphoric acid solution

the UV detection wavelength was at 440 nm (for crocin-Ⅰ and crocin-Ⅱ). Result: There were a good linear relationship between the concentration of icariin

baohuoside I and peak area value when the concentrations of icariin and baohuoside I were within the range of 0.054 6-1.092 μg (r=0.999 7)

0.076 2-1.524 μg (r=0.999 1). The average recovery were 97.56% (RSD 0.94%) and 99.17% (RSD 0.77%). There were a good linear relationship between the concentration of crocin-Ⅰ

crocin-Ⅱ and peak area value when the concentrations of crocin-Ⅰ and crocin-Ⅱ were within the range of 0.102 8-2.056 μg (r=0.999 4)

0.060 4-1.208 μg (r=0.999 8). The average recovery were 97.54% (RSD 0.90%) and 96.91% (RSD 1.27%). Conclusion: The method is accurate

sensitive and reproducible

it may be used in the determination of icariin

baohuoside I

crocin-Ⅰ and crocin-Ⅱ in HCJ.

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