HPLC测定复方肝炎颗粒中槲皮素和山奈素的含量

梁国华; 梁晓琪

doi:10.11653/syfj2013240090

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HPLC测定复方肝炎颗粒中槲皮素和山奈素的含量

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- HPLC测定复方肝炎颗粒中槲皮素和山奈素的含量
- Determination of Content of Quercetin and Kaempferol in Compound Ganyan Granules by HPLC
- 中国实验方剂学杂志 2013年19卷第24期页码：90-92
- 作者机构：
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- 基金信息：
  
  广西不良反应药物质量考察项目（桂科攻10124001A-66）
- DOI：10.11653/syfj2013240090
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- 纸质出版日期：2013
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梁国华, 梁晓琪. HPLC测定复方肝炎颗粒中槲皮素和山奈素的含量[J]. 中国实验方剂学杂志, 2013,19(24):90-92.

LIANG Guo-hua, LIANG Xiao-qi. Determination of Content of Quercetin and Kaempferol in Compound Ganyan Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 90-92.
梁国华, 梁晓琪. HPLC测定复方肝炎颗粒中槲皮素和山奈素的含量[J]. 中国实验方剂学杂志, 2013,19(24):90-92. DOI： 10.11653/syfj2013240090.

LIANG Guo-hua, LIANG Xiao-qi. Determination of Content of Quercetin and Kaempferol in Compound Ganyan Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 90-92. DOI： 10.11653/syfj2013240090.

摘要

目的：高效液相法测定复方肝炎颗粒中槲皮素和山奈素的含量。方法：采用HPLC，样品经80%甲醇超声、酸水解后，采用色谱柱C18 （4.6 mm× 250 mm，5 μm），甲醇-0.4%磷酸（50:50）为流动相，流速1.0 mL·min-1，检测波长360 nm，柱温30℃。结果：槲皮素在0.050 1~0.501 μg（r=0.999 9）和山奈素在0.024 2~0.242 μg（r=0.999 5）均呈良好线性关系；槲皮素和山奈素加样回收率分别为99.69%（RSD 0.22%），99.30%（RSD 0.82%）（n=9）。结论：该法操作简便﹑结果准确、重复性好﹑结果准确可靠，可用于复方肝炎颗粒的质量控制要求。

Abstract

Objective: To establish a HPLC method for quantification of quercetin and kaempferol in compound Ganyan granules. Method: Samples were extracted in 80% methanol with ultrasonic process and hydrolyzed for 1 h. The HPLC was performed on an ODS-C18column (4.6 mm×250 mm

5 μm) with methanol-0.4% H3PO4 (50:50) as mobile phase. The flow rate was 1.0 mL·min-1 and the column tempreture was kept at 30℃. The eluate was detected at 360 nm. Result: The linear ranges of quercetin and kaempferol were 0.050 1-0.501 μg (r=0.999 9) and 0.024 2-0.242 μg (r=0.999 5) respectively. The average recoveries of quercetin and kaempferol were 99.69%(RSD 0.22%) and 99.30%(RSD 0.82%). Conclusion: The method can be used for quality control of Ganyan granules. It is simple

rapid and accurate for simultancous determination of two constituents in compound Ganyan granules.

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