Objective: To analyze fat-soluble constituents in flowers of Tibetan medicinal plants of three Gentiana species

G. dendrologi

G. straminea and G. tibetica. Method: The fat-soluble components were extracted by Soxhlet extraction

separated and identified using GC-MS. The relative content of each component was determined by area normalization. Result: Thrity-eight

thrity-five and twenty-seven compounds from G. dendrologi

G. straminea and G. tibetica were identified respectively. The ratios of identification of total compounds were 94.31%

83.09% and 80.84% respectively. There are some differences among three Gentiana

the main ingredients being alkane (59.33%)

ethanol (14.53%)

and organic acid (6.43%) and ketones (3.84%) in flowers of G. dendrologi

alkane (28.24%)

ethanol (17.51%)

and organic acid (8.09%) and esters (7.55%) in G. straminea

and alkane (24.79%)

ethanol (5.79%)

and alkaloids (17.03%)

quinones (16.60%) and esters (2.78%) in G. tibetica G. tibetica showed different pharmacological activity owing to different chemical compositions. Conclusion: By analyzing and evaluating their fat-soluble components in three Gentiana Species

the scientific data can be provided for exploitation of Gentiana resources.