Objective: To establish HPLC fingerprint of Wenshen Jiangzhuo powder. Method: ANGLA Promosil C18 column (4.6 mm×250 mm

5 μm) was used with the column temperature kept at 30℃;the detection wavelength was set at 224 nm

and the injection volume was 10 μL

and a gradient solution of acetonitrile and H2O (acidified to 0.1% with phosphoric acid). Result: Most peaks could be separated preferably

and the RSD of the relative relation time of ten batches of Wenshen Jiangzhuo powder was lower than 1%

fourteen common peaks were obtained in the fingerprint of ten batches of Wenshen Jiangzhuo powder by peaks match with Similarity Evaluation System for Chromatographic Fingerprint of TCM. The similarity of fingerprints was calculated more than 0.93. Conclusion: The established HPLC fingerprint has desirable accuracy

repeatability and stability

it could be used as a method of quality control and provide evidence for the research of effective components of Wenshen Jiangzhuo powder.