Objective: To establish a HPLC characteristic fingerprints for the determination of the roots and stems of Berchem lineata. Method: The characteristic fingerprints of 24 batches of roots and stems of B. Lineata and B. floribunda

which were belong to different kinds and collected from different places

were established by HPLC. A chemical pattern recognition method was constructed by using cluster analysis and principal components analysis(PCA)method. Result: 11 common peaks were found in the HPLC characteristic fingerprints. 24 samples were divided into two branches. 9 batches of roots of B. Lineata and B. floribunda clustered to a clade. 3 roots of B. floribunda

12 stems of B. Lineata and B. floribunda were first clustered together as a sister clade to roots of B. Lineata and B. floribunda. Conclusion: The established method can be used for quality control of the roots and stems of B. lineata.