Objective: To study the inhibitory effects of modified Wuzi Yanzong prescription (MWP) on BV-2 microglial cells induced by lipopolysaccharides(LPS) and the potential mechanism. Method: BV-2 microglial cells were cultured in vitro and stimulated by LPS (1 mg·L-1).The cells were divided into three groups:blank control group

model group and MWP treatment group. The gradient 50

100

200 mg·L-1three concentrations were used in the treatment group. The expression of inflammatory factor nitric oxide(NO)

protein inducible nitric oxide synthese(iNOS) and cyclooxygenase(COX-2)

the translocation of nuclear factor-κB (NF-κB)

nicotinamide adenosine denucleotide hydrophosphori(NADPH) oxidase activity and reactive oxygen species were tested after treating the cells by LPS and MWP. Result: MWP could decrease the release of NO and expression of iNOS

COX-2 protein with a dose-dependent manner

the nuclear translocation of NF-κB was inhibited by MWP too. In addition

MWP could decrease the NADPH oxidase activity with a dose-dependent manner and significantly reduced the generation of ROS. Conclusion: MWP can significantly inhibit the inflammatory response of BV-2 microglial cells induced by LPS via suppression of NF-κB signaling pathway and NADPH oxidase-mediated oxidative stress.