Objective: To compare the neuroprotective effect of different extracts of Angelica pubescens on SH-SY5Y cells injury induced by hydrogen peroxide (H2O2) so as to screen the most pharmacologically active extracts. Method: Different extracts were obtained by means of reflux extraction

solvents extraction or silica gel column chromatography. Pro-incubation of different extracts on SH-SY5Y cells for 6 h prior to co-treat with 250 μmol·L-1 H2O2 for 24 h

the cell viability were observed by MTT assay. Meanwhile

the activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA) was determined.The expression of brain-derived neurotrophic factor (BDNF) and nerve factor-3 (NT-3) by immunofluorescence was measured. Result: All extracts showed the protective effect against decrease of cell viability induced by H2O2 and there was dose-effect relationship except the extract of petrdeum ether(PE) These extracts enhanced SOD activity and lowered H2O2-induced MDA release. Therefor

they exhibited the similar effect of anti-oxidation;According to increasing the level of BDNF and NT-3(94.5% and 97.5% vs. control

relatively)

we found that the PE-EtOAc extract was better neuroprotective than others. Conclusion: The different extracts of A. pubescens show some protective effect on H2O2 induced SH-SY5Y cells injury and the PE-EtOAc extract has the most pharmacologically activity.