HUANG Lei, XIAO Wen-juan, LIU Chao, et al. Effect of Three Heavy Metals on Cell Suspension Cultures of Biomass[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 226-229.
HUANG Lei, XIAO Wen-juan, LIU Chao, et al. Effect of Three Heavy Metals on Cell Suspension Cultures of Biomass[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 226-229. DOI: 10.11653/syfj2013240226.
Objective: The cell suspension cultures of Sorbus aucuparia (SASC) was used to study the effect of abiotic elicitors-heavy metals on the SASC biomass. Method: The different concentrations of lanthanum chloride
copper nitrate
lead nitrate solution were prepared to treat the SASC
an electronic balance was used for measurement of Biomass of SASC. Result: The cell growth was stimulated by low concentration of lanthanum chloride but inhibited by high concentration of lanthanum chloride
the effect on cell growth was similar to Hormesis phenomenon
optimal stimulating concentration was 0.01 mg·L-1;low concentration of copper nitrate inhibited cell growth but high concentrations stimulated cell growth
after treated with 0.05 mg·L-1 copper nitrate the cell biomass was the lowest;substantially all concentrations of lead nitrate stimulated cell growth
the optimal stimulating concentration was 0.5 mg·L-1. Conclusion: Heavy metals affect the SASC biomass
and its role in the extent and manner may be related to elicitors' type and concentration.