Objective: To observe the effect of Xiaoaishu(XAS) and serum containing XAS on proliferation and migration of human cervical carcinoma Hela cell in vitro

and investigate the mechanism. Method: The morphology of Hela cell was observed by inverted microscope. Thiazolyl blue (MTT) assay was used to detect Hela cell proliferation. Migration ability of Hela cells was measured through transwell assay. The carbohydrate antigen 125 (CA125) level of Hela cell was measured by enzyme linked immunosorbent assay (ELISA). Real time RT-PCR was used to detect the expression of vascular endothelial growth factor (VEGF) mRNA. Result: The morphology of Hela cell was changed after XAS treatment

the main expressions of Hela cell consisted in decreased cell diopter

turned round and small. The proliferation and migration of Hela cells was significantly inhibited by XAS and serum containing XAS. The level of CA125 and the expression of VEGF mRNA were decreased after XAS treatment

and XAS combined with Cisplatin had a better effect. Conclusion: XAS and serum containing XAS had obvious effect against the proliferation and migration of Hela cell

which showed much higher superiority to the single medication group combined with cisplatin

and it's mechanism may be related with the reduction of CA125 level and VEGF mRNA expression.