重组蜂毒肽对C6胶质瘤细胞的抑制作用及对 VEGF,Flt-1,MMP-9表达的影响

姚兴军; 王岳华; 侯文仲; 邓光策; 曾敏敏; 关北漩

doi:10.11653/syfj2013240267

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重组蜂毒肽对C6胶质瘤细胞的抑制作用及对 VEGF,Flt-1,MMP-9表达的影响

更新时间：2024-01-04

- 重组蜂毒肽对C6胶质瘤细胞的抑制作用及对 VEGF,Flt-1,MMP-9表达的影响
- Effect of Recombinant Melittin on Inhibition of Glioma C6 Cell Growth and the Expression of VEGF, Flt-1, MMP-9
- 中国实验方剂学杂志 2013年19卷第24期页码：267-269
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- DOI：10.11653/syfj2013240267
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- 纸质出版日期：2013
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姚兴军, 王岳华, 侯文仲, 等. 重组蜂毒肽对C6胶质瘤细胞的抑制作用及对 VEGF,Flt-1,MMP-9表达的影响[J]. 中国实验方剂学杂志, 2013,19(24):267-269.

YAO Xing-jun, WANG Yue-hua, HOU Wen-zhong, et al. Effect of Recombinant Melittin on Inhibition of Glioma C6 Cell Growth and the Expression of VEGF, Flt-1, MMP-9[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 267-269.
姚兴军, 王岳华, 侯文仲, 等. 重组蜂毒肽对C6胶质瘤细胞的抑制作用及对 VEGF,Flt-1,MMP-9表达的影响[J]. 中国实验方剂学杂志, 2013,19(24):267-269. DOI： 10.11653/syfj2013240267.

YAO Xing-jun, WANG Yue-hua, HOU Wen-zhong, et al. Effect of Recombinant Melittin on Inhibition of Glioma C6 Cell Growth and the Expression of VEGF, Flt-1, MMP-9[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 267-269. DOI： 10.11653/syfj2013240267.

摘要

目的：探讨重组蜂毒肽作用于大鼠C6胶质瘤细胞后，对细胞生长的抑制作用及对血管内皮生长因子（VEGF）、血管内皮生长因子受体（Flt-1）、基质金属蛋白酶-9（MMP-9）表达的影响。方法：对大鼠C6胶质瘤细胞进行体外培养后，分为对照组及重组蜂毒肽低、中、高剂量治疗组，治疗组分别给予相应浓度的重组蜂毒肽（20，50，80 mg·L-1），采用MTT法于24，48，72 h分别测定C6胶质瘤细胞的生长情况；并采用Western blot法测定不同浓度重组蜂毒肽作用72 h后C6胶质瘤细胞VEGF，Flt-1，MMP-9的表达情况。结果：与对照组比较，不同浓度的重组蜂毒肽作用于细胞24 h后，细胞生长情况无明显变化，但48，72 h后，低、中、高浓度治疗组的细胞的增殖活性均有不同程度的降低、生长抑制率增高（P<0.01或P<0.05）；同时中、高浓度重组蜂毒肽作用72 h后，胶质瘤细胞的VEGF，Flt-1，MMP-9表达水平均有不同程度的降低（P<0.01或P<0.05）。结论：重组蜂毒肽对C6胶质瘤细胞的生长和VEGF，Flt-1，MMP-9的表达均有明显的抑制作用，因此重组蜂毒肽可能通过抑制VEGF，Flt-1，MMP-9的表达来抑制胶质瘤的生长。

Abstract

Objective: To explore the effect of recombinant melittin on the glioma C6 cells and the expression of vascular endothelial growth factor (VEGF)

vascular endothelial growth factor receptor-1 (Flt-1) and matrix metalloproteinase-9 (MMP-9) in the cells. Method: Glioma C6 cells were separated into control group and 3 treatment groups

then 20

80 mg·L-1 recombinant melittin was given. MTT method was used to evaluate the inhibitory effects of recombinant melittin with different doses after 24

72 h on glioma C6 cell growth;the proteins expression of VEGF

Flt-1 and MMP-9 were determined by Western blot analysis. Result: Compared with control group

no significant change was found on glioma C6 cell growth after treated with recombinant melttin for 24 h in each treatment groups

but significantly inhibition of the glioma C6 cells proliferation was observed after treated with recombinant melttin for 48 h and 72 h in each groups (P<0.01 or P<0.05). The assay of Western blot showed that the proteins expression of VEGF

Flt-1 and MMP-9 in glioma C6 cells induced by recombinant melittin were significantly down-regulated when the cells were treated with 50

80 mg·L-1 recombinant melittin for 72 h. Conclusion: Recombinant melittin can inhibit the glioma C6 cell growth and the expression of VEGF

Flt-1 and MMP-9 in the cells

so recombinant melittin maybe can inhibit the growth of glioma by affect expression of VEGF

Flt-1 and MMP-9.

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