复方三七纳米颗粒对颅脑损伤大鼠血脑屏障和 血清TNF-和IL-6的影响

王珠; 赵雅宁; 李建民; 黄海玲; 刘文倩

doi:10.11653/syfj2013240270

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复方三七纳米颗粒对颅脑损伤大鼠血脑屏障和血清TNF-和IL-6的影响

更新时间：2024-01-04

- 复方三七纳米颗粒对颅脑损伤大鼠血脑屏障和血清TNF-和IL-6的影响
- Effects of Compound Notogin Sengnano Granules on Blood Brain Barrier, Tumor Necrosis Factor- and Interleukin-6 after Brain Injury in Rats
- 中国实验方剂学杂志 2013年19卷第24期页码：270-273
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北省科技厅支撑项目（202761020）
- DOI：10.11653/syfj2013240270
  中图分类号：
- 纸质出版日期：2013
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王珠, 赵雅宁, 李建民, 等. 复方三七纳米颗粒对颅脑损伤大鼠血脑屏障和血清TNF-和IL-6的影响[J]. 中国实验方剂学杂志, 2013,19(24):270-273.

WANG Zhu, ZhAO Ya-Ning, LI Jian-min, et al. Effects of Compound Notogin Sengnano Granules on Blood Brain Barrier, Tumor Necrosis Factor- and Interleukin-6 after Brain Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 270-273.
王珠, 赵雅宁, 李建民, 等. 复方三七纳米颗粒对颅脑损伤大鼠血脑屏障和血清TNF-和IL-6的影响[J]. 中国实验方剂学杂志, 2013,19(24):270-273. DOI： 10.11653/syfj2013240270.

WANG Zhu, ZhAO Ya-Ning, LI Jian-min, et al. Effects of Compound Notogin Sengnano Granules on Blood Brain Barrier, Tumor Necrosis Factor- and Interleukin-6 after Brain Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(24): 270-273. DOI： 10.11653/syfj2013240270.

摘要

目的：观察复方三七纳米颗粒对脑创伤后大鼠血脑屏障、肿瘤坏死因子-α（TNF-α）和白介素-6（IL-6）的影响作用。方法：用Feeney自由落体撞击法建立成年雄性大鼠脑创伤模型，96只实验动物随机分为空白对照组、创伤模型组、复方三七纳米颗粒低、高剂量组（0.6，2.4 g·kg-1），每组24只大鼠。第1，3，5天参照ZeaLonga法，对各组实验大鼠进行神经功能缺损评分；之后经腹主动脉穿刺取血，用双抗体夹心酶联免疫吸附法（ELISA）测量各组不同时间点大鼠（n=8）血清肿瘤坏死因子-α（TNF-α）和白介素-6（IL-6）含量的变化；之后处死大鼠，取造模损伤部位脑组织（约4 mm×4 mm×3 mm）称重计算脑组织含水量，观察用药后各时间点大鼠脑水肿情况。结果：与空白对照组比较，模型组神经功能缺损评分升高，脑组织含水量、TNF-α和IL-6含量均增加；与模型组比较，复方三七纳米颗粒干预组中神经功能缺损评分降低，脑组织含水量、TNF-α和IL-6含量均降低，并且随着剂量的增加上述变化显著（P<0.05）。结论：复方三七纳米颗粒对大鼠脑创伤后有保护作用，与TNF-α的含量降低和IL-6含量增加有关。

Abstract

Objective: To investigate the effects of the compound Notogin Sengnano granules on blood brain barrier

tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in rats after traumatic brain injury. Method: The trauma model were established according to the Feeney free-fall impact brain injury using adult male rats.The nine-six rats were divided into four groups:control group

model group

low-dose compound Notogin Sengnano granules group(0.6 g·kg-1)and high-dose compound Notogin Sengnano granules group(2.4 g·kg-1). The rats in each group were scored according to the ZeaLonga neural function score.At the 1

5 d

blood samples were collected to detect detect(TNF-α) and IL-6 by ELISA.The brain tissue were collected for determination of water content. Result: Compared with control group

in model group the neural function defect score was increased

the brain water contents significantly increased

the contents of TNF-α and IL-6 content were increased. Compared with model group

in compound Notogin Sengnano gragules group

the neural function defect score

the brain water contents

TNF-α and IL-6 levels were decreased

significantly decreased(P<0.05). Conclusion: The compound Notogin Sengnano granules can promote the recovery of neural function and reduce the content of the TNF-α and IL-6 in rats after the traumatic brain injury.

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