Objective: To optimize extraction process of airtight Chinese medicine composite dressings. Method: With contents of ginsenoside Rg1 and Rb1

extract yield as evaluation indexes

extraction times was optimized by single factor test

orthogonal design was adopted to optimize extraction technology of airtight Chinese medicine composite dressings by taking the amount and content of ethanol

extraction time as factors.Contents of ginsenoside Rg1 and Rb1 were determined by HPLC

mobile phase of water(A)-acetonitrile(B) with a gradient elution program(0-10 min

81% A;10-38 min

81%-79% A;38-90 min

79%-66% A)

detection wavelength 203 nm. Result: Effect of each factor on extraction technology was in order of ethanol concentration>extraction time>ethanol amount.Optimum extraction process were as follows:extracted 2 times with 8-fold the amount of 70% ethanol

2 hours per time;Average extract yield was 25.45%

average concentration of ginsenoside Rg1 and Rb1 were 0.46

0.37 g·L-1. Conclusion: This established method was accurate and reproducible

optimized extraction process was stable

reasonable and practicable

this study could provide a reference for secondary development of clinical prescriptions.