Objective: To establish the quality standard of monomer combination drug of Shenqi Yizhi

which could be appliable to establishing the foundation of its quality standards. Method: Due to different kinds of components in the drug

different extraction methods for sample preparation was adopted herein

several developer systems were investigated in this TLC study. A HPLC method was established for the determination of four flavonoids of this monomer combination drug. Result: TLC indentification methods of ferulaic acid

gastrodine

epimedium total flavonoids

ginseng total saponins and astragalus total saponins in monomer combination drug of Shenqi Yizhi were finally established respectively. Linear equations

sensitivities

precisions

stabilities

etc of four flavonoids of this drug under the HPLC were all in line with requirements of HPLC assay. Conclusion: The reported methods are simple

practical

sensitive

accurate

specific and reproducible

which can be used as quality control method of monomer combination drug of Shenqi Yizhi.