Objective: To establish a HPLC fingerprint analysis method for the quality control of Abrus mollis. Method: A HPLC method was adopted. Chromatographic assay was performed on a Lichrospher C18column (4.6 mm×250 mm

5 μm) at room temperature with methanol-0.1% TFA (35:65) as the mobile phase. The flow rate was 0.8 mL·min-1 and the detection wavelength was set at 272 nm. 10 batches samples of A. mollis were determined for similarity comparison. Result: A HPLC fingerprint analysis method of A. mollis was established. 9 common peaks were assigned

and 4 peaks of them were confirmed by comparing with reference standards. Similarity of 10 batches of A. mollis was more than 0.98. Conclusion: This method was rapid

accurate and reproducible

it could provide a reference for quality evaluation and control of A. mollis.