Objective: To study Buzhong Yiqi decoction‘Yiqi Shengyang' compatibility mechanism based on metabonomics. Method: The nuclear magnetic resonance spectroscopy (1H-NMR) was used to establish the rat serum metabolic fingerprinting techniques.The orthogonal partial least-squares discriminant was applied for analysis of metabolite profiling difference between model group and Buzhong Yiqi decoction group

increased Cimicifugae Rhizoma-Bupleuri Radix dosage group

Buzhong Yiqi without CⅡcifugae Rhizoma-Bupleuri Radix group

and through the variable importance in projection (VIP) biomarkers in serum were selected

the markers were compared to find the differences. Result: the moderating effect of Buzhong Yiqi decoction on spleen deficiency syndrome was reflected in the way of energy metabolism

glucose metabolism

lipid metabolism

amino acid metabolism regulation revision

while increased Cimicifugae Rhizoma-Bupleuri Radix dosage group

Buzhong Yiqi without Cimicifugae Rhizoma-Bupleuri Radix group improved three big nutrients metabolism to a certain extent in models group rats

but failed to restore the rat model' energy metabolism fundamentally

still in anaerobic respiration. Conclusion: Buzhong Yiqi decoction has adjustment effect on spleen deficiency of the strongest

mainly reflect in the recovery of amino acid metabolism and energy metabolism.