Objective: To observe the anti-inflammatory and analgesic effects of Zhengshi Fumigation and Washing Medicine Ⅰ (ZSFWM Ⅰ) and its effect on the levels of interleukin 1β(IL-1β) and prostaglandin E2(PGE2) in rats with acute soft tissue injury. Method: Adult SD rats and KM mice were divided into control group

ZSFWM Ⅰ (0.013

0.026

0.053 g·mL-1) group

Yunnan Baiyao tincture (0.01 g·mL-1) group randomly. Mice pain model induced by hot plate and acetic acid

rats acute and subacute inflammation including plantar edema induced by albumen and granuloma induced by cotton ball were made to observe the anti-inflammatory and analgesic effects of ZSFWM Ⅰ. Acute soft tissue injury rat model was made and divided into different groups randomly

ZSFWM Ⅰ or Yunnan Baiyao tincture was given to the stricken animals. Injury tissue were taken from the animals after strike 2

4

6 days

the IL-1β and PGE2 contents were measured through ELISA and western blot. Result: ZSFWM Ⅰ could significantly mitigate the pain response induced by heat and acetic acid in mice(P<0.05)

it also could relieve the plantar edema and inhibit the proliferation of granulation tissue in rats(P<0.05). In addition

ZSFWM Ⅰ could significantly decrease the levels of IL-1β and PGE2 in injury soft tissue (P<0.01). Conclusion: ZSFWM Ⅰ has obvious anti-inflammatory and analgesic effects. It can inhibit the increase of IL-1β and PGE2 expression in injured soft tissue

which may be one of the mechanisms that ZSFWM Ⅰ can the treat acute soft tissue injury.