Objective: To optimize extraction process of total flavonoids from Selaginellae Herba against hepatocarcinoma cells SMMC-7721. Method: Taking extraction time and times

the amount and concentration of ethanol as factors

result of orthogonal test was adopted to optimize weight coefficient ratio of amentoflavone and total flavonoids extraction amounts in which inhibition rate of human hepatoma SMMC-7721 cells was taken as an indicator

contents of effective components and pharmacodynamic indicators were analyzed with Pearson correlation coefficient by SPSS 17.0 software.The content of amentoflavone was determined by HPLC

mobile phase of acetonitrile-0.5% acetic acid water(33:67)

detection wavelength 269 nm. Result: The best extraction conditions were as follows:reflux extracted 3 h with ten times the amount of 70% ethanol;weight coefficients of contents of amentoflaveone and total flavonoids were 0.7 and 0.3

indicating determination of effective ingredients and inhibition rate had a certain correlation. Conclusion: Taking contents of active ingredients instead of efficacy as the index of orthogonal test was scientific and reasonable;Optimized process was stable and feasible

it could provide new ideas for extraction of active ingredients from traditional Chinese medicine.