Objective: To optimize separation and purification technology of icariin and icariside Ⅱ from Epimedii Folium by macroporous adsorption resin. Method: Taking adsorption capacity and desorption rate of icariin and icariside Ⅱ as indexes

macroporous resin models were screened by static adsorption-elution test

single factor tests were adopted to investigate adsorption and elution conditions. Result: DM301 macroporous resin was the most suitable one for purification of icariin and icariside Ⅱ

its optimum adsorption conditions were as follows:the concentration of sample solution 7.45 g·L-1

sample flow rate 3 BV·h-1;Elution conditions of icariin was eluting with 4 BV 45% ethanol at 1.5 BV·h-1;Elution conditions of icatiside Ⅱ was eluting with 5 BV 60% ethanol at 1.5 BV·h-1;Under these conditions

purities of icariin and icariside Ⅱ were 56.23% and 67.41%

respectively. Conclusion: These established process conditions could be adopted to separate and purify icariin and icariside Ⅱ at the same time with good stability

high yield and low production cost

which were suitable for industrial production.