Objective: To optimize separation and purification process of total saponins from Anredera cordifolia by macroporous resin. Method: The content of total saponins was determined by UV.Model of macroporous resin was screened by static adsorption-elution test;Orthogonal test was adopted to optimize separation and purification process of total saponins from A. cordifolia by taking dynamic adsorption capacity and elution rate as indexes. Result: D101 macroporous resin was selected

its optimum separation and purification process conditions were determined as follows:the concentration of sample solution 0.1 g·mL-1

adsorption velocity 1 BV·h-1

ratio of diameter-high of cation resin column 1:10

quality proportion of crude drug-resin 0.2 g·mL-1

ethnol concentration 40%

elution velocity 2 BV·h-1

eluent volume 8 BV;Purity of total saponins from A. cordifolia under these conditions could reach above 50%. Conclusion: Optimized separation and purification process was reasonable and feasible

it could easily separate and purify total saponins from A. cordifolia.