Objective: To develope a near-infrared quantitative method for rapid determination of total glycosides in countercurrent extracting process of Gardeniae Fructus. Method: UV was used as a reference method to determine the content of total glycosides in Gardeniae Fructus

a rapid on-line determination method of total geniposides was established by NIR transmission method and partial least squares method.Sampling method for transmission acquisition

collection interval 4 000-10 000 cm-1

resolution 8.0 cm-1

numner of scans 64 times

empty door attenuation

gain of 1

temperature at (25±2) ℃

relative humidity 45%-50%. Result: Correlation coefficients and calibration root-mean-square error of calibration model for total glycosides in countercurrent extract of Gardeniae Fructus were 0.993 and 0.179

respectively.By external validation

prodiction root-mean-square error of calibration model was 0.145

correlatiaon coefficient of regression equation between the actual value and the predicted value was 0.991. Conclusion: NIR could be used as a kind of accurate

rapid and on-line detection method for determination of active ingredients in extracting process of traditional Chinese medicine.