Objective: An analytical method was developed for simultaneously determining alisol C 23-acetate

alisol A

alisol B

alisol B 23-acetate four triterpenoids in the rhizoma of Alisma orientalis. Method: The assay was performed on a Ultimate XB-C18 column (4.6 mm×250 mm

5 μm) with the mobile phase consisting of acetonitrile-water (65:35) at a flow rate of 1.0 mL·min-1. The column temperature was at 30 ℃ and the optimum detection wavelength of DAD was set at 245 nm. The drift tube and atomizer temperature of ELSD were 60

55 ℃

with a carrier gas (N2) pressure of 20 psi. Result: The linear range of alisol C 23-acetate

Alisol A

alisol B and alisol B 23-acetate were 1.869-29.90 mg·L-1(r=0.999 8)

3.731-74.62 mg·L-1(r=0.999 7)

8.653-138.4 mg·L-1(r=0.999 9)

4.832-77.31 mg·L-1(r=0.999 5)

respeltively. The average recoveries (n=6) were 98.78%(RSD 2.63%)

98.05%(RSD 2.72%)

98.26%(RSD 2.86%) and 97.65%(RSD 2.95%) respectively. Conclusion: The HPLC method is simple

rapid and accurate. This HPLC-DAD-ELSD verified method can be used for quantitative analysis of multi-component of Rhizoma Alismatis and provides a novel approach for evaluation of the quality of Rhizoma Alismatis comprehensively.