Objective: To establish the HPLC fingerprint of Arnebiae Radix smashing tissue extraction with ethanol and reflect the chemical information comprehensively and provide evidence for quality control. Method: HPLC was used on a Kromasil 100-5 C18 (4.6 mm×250 mm

5 μm) by gradient elution with solvent system composed of water-formic acid (300:0.5)-acetonitrile

detection wavelength was 275 nm

the flow rate was 1 mL·min-1

the column temperature was set at 25 ℃. Result: HPLC fingerprint of Arnebiae Radix smashing tissue extraction with ethanol was established

which showed 13 characteristic peaks from 10 batches of crude drug. Among them

the No 4

6

10 peaks were respectively identified as shikonin

acetylshikonin

and β

β'-dimethylacryl alkannin. Conclusion: The method is simple and has good precision

reproducibility and stability

and can be applied to the quality control the Arnebiae Radix smashing tissue extraction with ethanol

also provide a quality assurance for further pharmacological studies and preparation research.