HPLC测定除痰止嗽丸中的桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素

李红燕; 张蕊; 冯晓川

doi:10.11653/syfj2014020078

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HPLC测定除痰止嗽丸中的桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素

更新时间：2024-01-04

- HPLC测定除痰止嗽丸中的桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素
- Determination of Platycodin D, Platycodin E, Praeruptorin A and Praeruptorin B in Chutan Zhisouwan by HPLC
- 中国实验方剂学杂志 2014年20卷第2期页码：78-82
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- 作者简介：
- 基金信息：
  
  北京博士后基金项目（2011M500494）
- DOI：10.11653/syfj2014020078
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- 纸质出版日期：2014
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李红燕, 张蕊, 冯晓川. HPLC测定除痰止嗽丸中的桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素[J]. 中国实验方剂学杂志, 2014,20(2):78-82.

LI Hong-yan, ZHANG Rui, FENG Xiao-chuan. Determination of Platycodin D, Platycodin E, Praeruptorin A and Praeruptorin B in Chutan Zhisouwan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 78-82.
李红燕, 张蕊, 冯晓川. HPLC测定除痰止嗽丸中的桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素[J]. 中国实验方剂学杂志, 2014,20(2):78-82. DOI： 10.11653/syfj2014020078.

LI Hong-yan, ZHANG Rui, FENG Xiao-chuan. Determination of Platycodin D, Platycodin E, Praeruptorin A and Praeruptorin B in Chutan Zhisouwan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 78-82. DOI： 10.11653/syfj2014020078.

摘要

目的：采用高效液相色谱法测定除痰止嗽丸（CTZSW）中桔梗皂苷D、桔梗皂苷E、白花前胡甲素和白花前胡乙素的含量。方法： Hypersil C18色谱柱（4.6 mm×150 mm，5 μm），流速1.0 mL·min-1，流动相乙腈-水（35：65）（桔梗皂苷D和桔梗皂苷E），ELSD漂移管温度105 ℃，载气（N2）流速2.6 SLPM·min-1；流动相乙腈-甲醇-水（40：30：30）（白花前胡甲素和白花前胡乙素），检测波长321 nm。结果：桔梗皂苷D和桔梗皂苷E分别在0.041 3~0.826 0 μg（r=0.999 7），0.079 7~1.594 0 μg（r=0.999 8）进样量与峰面积呈良好的线性关系，平均加样回收率分别为98.32%，98.94%，RSD（n=6）分别为0.80%，0.93%；白花前胡甲素和白花前胡乙素分别在0.154 9~3.098 0 μg（r=0.999 4），0.051 7~1.034 0 μg（r=0.999 8）进样量与峰面积呈良好的线性关系，平均加样回收率分别为98.72%，99.15%，RSD（n=6）分别为0.86%，0.95%。结论：该方法测定结果准确、灵敏、重复性好；可用于除痰止嗽丸的质量评价。

Abstract

Objective: To develop an HPLC method for determination of the content of platycodin D

platycodin E

praeruptorin A and praeruptorin B in Chutan Zhisouwan. Method: A Hypersil C18 column was used as the chromatographic column. The flow rate was 1.0 mL·min-1. The mobile phase consisted of acetonitrile-water (35:65) for platycodin D and platycodin E

the temperature of drift tube was set at 105 ℃;the gas flow (N2) was set at 2.6 SLPM·min-1.The mobile phase consisted of acetonitrile-methanol-water (40:30:30) for praeruptorin A and praeruptorin B

The UV detection wavelength was set at 321 nm. Result: Platycodin D and platycodin E were linear within the range of 0.041 3-0.826 0 μg (r=0.999 7) and 0.079 7-1.594 0 μg (r=0.999 8) respectively. The average recoveries were 98.32% (RSD 0.80%) and 98.94% (RSD 0.93%). Praeruptorin A and praeruptorin B were linear within the range of 0.154 9-3.098 0 μg (r=0.999 4) and 0.051 7-1.034 0 μg (r=0.999 8) respectively. The average recoveries were 98.72% (RSD 0.86%) and 99.15% (RSD 0.95%). Conclusion: The method was accurate

sensitive and reproducible

which may be used in the determination of platycodin D

platycodin E

praeruptorin A and praeruptorin B in CTZSW.

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