Objective: To study the effects of Xiangfu Siwu decoction on the blood

nerve endocrine immune (NEI) system in female rats with Qi stagnation and blood stasis syndrome. Method: The female SD rats model with Qi stagnation and blood stasis syndrome were prepared by giving adrenaline(Adr) and chronic unpredicted stress

while the medicine groups were treated with Xiangfu Siwu decoction. ELISA and immunohistochemistry assay were employed to detecte contents of interlerkin-6(IL-6)

leukotrienes 4(LTB4)

estradiol(E2)

progesterone(P)

prostaglandin E2(PGE2)

nitric oxide(NO) in the serum and β-endorphin in hypothalamus before and after the treatment. PLS-DA(Patrial least squares discriminant analysis)method was used to analyze the above factors. Result: Compared with control group

the model group had significantly higher levels on hemorheology and significantly lower levels on thrombin time(TT) and activated partial thromboplastin time(APTT)

while the model group had no obvious changes on prothrombin time(PT) and fibrinogen;the model group had significantly higher levels on the content of IL-6

P in the serum

and significantly lower levels on the contents of E2

PGE2

NO in the serum

while the model group had no obvious changes on the content of LTB4.Xiangfu Siwu decoction had a significant effect on improving the model with Qi stagnation and blood stasis syndrome. Compared with control group

the medicine groups had no significant difference. Through a multi-index analysis with PLS-DA

the most important X variables were E2

PGE2

P

TT

horizontal movement

area

vertical movement

integral optical density(IOD)

30 s-1 whole blood riscosity

residence time

plasma viscosity. Conclusion: The therapeutic effects of Xiangfu Siwu decoction on many diseasesmay be mediated by modulating multi-level of blood

nerve

immune

endocrine system and keeping internal environment homeostasis.