Objective: To investigate effect of serum containing Sarcandrae compound on proliferation

telomerase activity and cellular apoptosis of HepG2 liver cancer cells. Method: Serum containing drugs was prepared by Administration of physiological saline

different concentrations of Sarcandrae compound and 5-fluoro-2

4(1H

3H)pyrimidinedion(5-FU). Consequently to rats. The serum containing drugswas used totreat HepG2 cells for 24

48

72 h. Then the effects of different drugs on proliferation

telomerase and apoptosis of HepG2 cells were observed by MTT

ELISA and Flow cytometry Annexin V-FITC/PI assay

respectively. Result: Compared to control group

proliferation and telomerase activities of HepG2 cells were inhibited with time-dependent manner by serum containing Sarcandrae compound significantly (P<0.05). All serum containing Sarcandrae compound could promote the apoptosis of HepG2 cells significantly. For middle dose group at 24

48

72 h the apoptosis rate was (14.2±0.9)%

(30.6±1.0)%

(61.6±1.5)%. Conclusion: Serum containing Sarcandrae compound can inhibit the proliferation and telomerase activity of HepG2 cells

and promote the apoptosis of HepG2 cells.