Objective: To investigate the mechanism of Qingre Jiedu prescription in treatment septic. Method: The septic model was induced by cecal ligation and puncture. The experimental rats were divided into five groups;control group

septic model group

Qingre Jiedu prescription treated group (24

12

6 g·kg-1). All animals were treaded via gastrogavage with distilled water and Qingre Jiedu prescription once a day for 3 days. The survival state was observed. The bacterial populations in experimental sepsis were determined by bacterial culture. And aquaporins(AQP) 2 and AQP8 were detected by western blot. Result: The fatality rate in model group was the highest (56%)

meanwhile all bacteria in ascites

lymph nodes and bloods were significantly higher than that in control group. For treaded group

the mortality and the serum biochemical parameter in the high dose group were significantly decreased. The AQP2 and AQP8 expression levels in all treated groups were decreased compared with that in model group

with a dose-dependent manner. In the high dose group

the number of pathogens in lymph nodes and blood was significantly lower than that in model group. Conclusion: Qingre Jiedu prescription can decrease bacterial translocation by reducing expression of AQPs to increase excreta.