Objective: To investigate the effect of Haoqin Qingdan decoction on the content of interleukin 8(IL-8) in serum and expression of IL-8

neclear factor-κB p65(NF-κB p65) in Gastric mucosa in Helicobacter pylori-associated gastritis(HAG) mice and explore its therapeutic mechanism for Helicobacter pylori(HP) associated gastritis. Method: HP standard strains (SS1) brinell broth liquid was used to establish the HAG mice model. Sixty healthy BALB/c mice were randomly devided into six group (n=10

each)

normal control group(given with equal NS)

model group (given with equal NS)

livzon weisanlian group(0.25 g·kg-1)

Haoqin Qindan decoction (4

8

16 g·kg-1) group. The mice in every group were administrated with drugs by oral gavage once a day. All mice were killed two weeks later

the pathological changes of each group were observed by the microscope

the contents of IL-8 in serum were detected by enzyme-linked immunoassay and the expression of IL-8

NF-κB p65 in gastric mucosa tissue were detected by immunohistochemistry. Result: Compared with model group

Haoqin Qingdan decoction significantly reduced the inflammation area and average blackness of gastric mucosa in HAG mice (P<0.01);the expression of IL-8 in serum of Haoqin Qingdan decoction groups were significantly lower than that in model group (P<0.01);the expression of IL-8

NF-κB p65 in gastric mucosa of Haoqin Qingdan decoction groups were significantly lower than that in model group (P<0.05). Conclusion: Haoqin Qingdan decoction can plays a significant role in the repair of gastric mucosal injury in HAG mice

its therapeutic mechanism may be related to inhibiting the activation of NF-κB p65 and degrading the expression of IL-8 in the gastric mucosa and serum.