化痰通络方对急性脑梗死大鼠溶栓后脑微血管内皮细胞构成蛋白基因表达的影响

张玉莲; 周震; 刘爽; 宋宛珊; 马妍; 田涛涛; 王凯

doi:10.11653/syfj2014020157

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化痰通络方对急性脑梗死大鼠溶栓后脑微血管内皮细胞构成蛋白基因表达的影响

更新时间：2024-01-04

- 化痰通络方对急性脑梗死大鼠溶栓后脑微血管内皮细胞构成蛋白基因表达的影响
- Effects of Huatan Tongluo on Gene Expression of Brain Microvascular Endothelial Cells in Rats with Acute Cerebral Infarction after Thrombolysis
- 中国实验方剂学杂志 2014年20卷第2期页码：157-162
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81072796）;天津市应用基础及前沿技术研究计划（10JCZDJC20500）
- DOI：10.11653/syfj2014020157
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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张玉莲, 周震, 刘爽, 等. 化痰通络方对急性脑梗死大鼠溶栓后脑微血管内皮细胞构成蛋白基因表达的影响[J]. 中国实验方剂学杂志, 2014,20(2):157-162.

ZHANG Yu-lian, ZHOU Zhen, LIU Shuang, et al. Effects of Huatan Tongluo on Gene Expression of Brain Microvascular Endothelial Cells in Rats with Acute Cerebral Infarction after Thrombolysis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 157-162.
张玉莲, 周震, 刘爽, 等. 化痰通络方对急性脑梗死大鼠溶栓后脑微血管内皮细胞构成蛋白基因表达的影响[J]. 中国实验方剂学杂志, 2014,20(2):157-162. DOI： 10.11653/syfj2014020157.

ZHANG Yu-lian, ZHOU Zhen, LIU Shuang, et al. Effects of Huatan Tongluo on Gene Expression of Brain Microvascular Endothelial Cells in Rats with Acute Cerebral Infarction after Thrombolysis[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(2): 157-162. DOI： 10.11653/syfj2014020157.

摘要

目的：观察化痰通络法对急性脑梗死大鼠重组组织型纤溶酶原激活剂（rt-PA）溶栓后脑微血管内皮细胞构成蛋白基因表达的影响，为化痰通络法防治急性脑梗死溶栓后缺血再灌注损伤提供实验依据。方法： 240只健康SD大鼠随机分为6组：假手术组、模型组、rt-PA组、化痰通络方低、中、高剂量联合rt-PA组（联合组），每组40只，采用自身栓子法制备大鼠大脑中动脉栓塞模型（MCAO），rt-PA组与化痰通络方联合rt-PA组于血栓注入后3 h一次性予以rt-PA溶栓治疗，后者联合给予低、中、高剂量（生药3.6，7.2，14.4 g·kg-1）化痰通络方灌胃治疗，每日2次，分别于造模后6，24，72 h，7 d 4个时点，应用RT-PCR法观察不同时相大鼠皮质及海马紧密连接蛋白Occludin，claudin-1，Zonula occludins protins-1（ZO-1）基因的表达。结果：在不同时点，各实验组在不同脑组织中Occludin，claudin-1，ZO-1基因表达量均在6 h最高，24 h最低，72 h，7 d数值逐渐升高，且联合组升高趋势更明显。与组内6 h时比较，rt-PA组、联合低、中、高剂量组Occludin，claudin-1，ZO-1基因表达量多于24，72 h（P < 0.05）。而同一时点与模型组相比，联合中、高剂量组各时点Occludin，claudin-1基因表达量均明显升高（P < 0.05）。而ZO-1基因表达量虽具有相似变化趋势，但仅在24，72 h，7 d差异具有统计学意义（P < 0.05）。结论：化痰通络方联合rt-PA溶栓，可通过调控微血管内皮细胞构成蛋白Occludin，claudin-1，ZO-1基因的表达，进而保护血脑屏障完整性，防治急性脑梗死溶栓后缺血再灌注损伤的发生与发展。

Abstract

Objective: To observe the effect of Huatan Tongluo therapy on gene expression of brain microvascular endothelial cells in rats with acute cerebral infarction after recombinant tissue-type plasminogen(rt-PA) thrombolysis

and provide experimental basis for preventive and therapeutict effects of Huatan Tongluo on ischemia-reperfusion injury induced by acute cerebral infarction after thrombolysis. Method: Two hundred and fouty healthy SD rats were randomly divided into six groups

sham operation group

model group

rt-PA group

rt-PA combined with Huatan Tongluo low dose group

rt-PA combined with Huatan Tongluo middle dose group

rt-PA combined with Huatan Tongluo high dose group. The middle cerebral artery occlusion(MCAO) rat model was prepared by autologous blood clots. rt-PA group and rt-PA combined with Huatan Tongluo groups used rt-PA thrombolytic therapy after thrombosis. The later were also given low

medium

high dose(3.6

7.2

14.4 g·kg-1) Huatan Tongluo prescription by gavage two times a day. Then observed the expression of occluding

claudin-1

ZO-1 in rat cortex and hippocampus by RT-PCR at 6

72 h and 7 days respectively. Result: In different phases of every experimental group

the expression of occluding

claudin-1

ZO-1 in different brain tissue were highest at 6 h

minimum at 24 h

increased gradually at 72 h and 7 days

and the combined with Huatan Tonglow groups increased more obviously. The expression of occluding

claudin-1

ZO-1 in rt-PA

low

middle

high dose groups at 6 h were significantly higher than that at 24

72 h(P<0.05). Compared the model group at the same time

the expression of occluding

claudin-1 in middle dose and high dose groups of Chinese traditional medicine were significantly increasedin each phase (P<0.05). While the ZO-1 gene expression had the similar trends

but significant difference only existed at the 24

72 h

7 d (P<0.05). Conclusion: Huatan Tongluo combined with rt-PA therapy can regulate the expression of occluding

claudin-1

ZO-1 in brain microvascular endothelial cells. So it can protect the integration of blood brain barrier

and have protective effects on ischemia reperfusion injury induced by acute cerebral infarction after thrombolysis.

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