响应面分析法优化桑叶中1-脱氧野尻霉素的稀酸乙醇浸提工艺

苏楠; 何羡霞; 冯青; 吴新荣

doi:10.11653/syfj2014030013

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响应面分析法优化桑叶中1-脱氧野尻霉素的稀酸乙醇浸提工艺

更新时间：2024-01-04

- 响应面分析法优化桑叶中1-脱氧野尻霉素的稀酸乙醇浸提工艺
- Optimization of Dilute Acid Ethanol Extraction Technology for 1-Deoxynojirimycin from Mori Folium by Response Surface Methodology
- 中国实验方剂学杂志 2014年20卷第3期页码：13-16
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省科技重点项目（2008A030201031）
- DOI：10.11653/syfj2014030013
  中图分类号：
- 纸质出版日期：2014
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苏楠, 何羡霞, 冯青, 等. 响应面分析法优化桑叶中1-脱氧野尻霉素的稀酸乙醇浸提工艺[J]. 中国实验方剂学杂志, 2014,20(3):13-16.

SU Nan, HE Xian-xia, FENG Qing, et al. Optimization of Dilute Acid Ethanol Extraction Technology for 1-Deoxynojirimycin from Mori Folium by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 13-16.
苏楠, 何羡霞, 冯青, 等. 响应面分析法优化桑叶中1-脱氧野尻霉素的稀酸乙醇浸提工艺[J]. 中国实验方剂学杂志, 2014,20(3):13-16. DOI： 10.11653/syfj2014030013.

SU Nan, HE Xian-xia, FENG Qing, et al. Optimization of Dilute Acid Ethanol Extraction Technology for 1-Deoxynojirimycin from Mori Folium by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(3): 13-16. DOI： 10.11653/syfj2014030013.

摘要

目的：优化桑叶中1-脱氧野尻霉素（1-DNJ）的稀酸乙醇浸提工艺。方法：采用HPLC-ELSD测定1-DNJ含量，流动相乙腈-水（90：10），ELSD漂移管温度40 ℃，载气流速3.5×105 Pa。以1-DNJ提取率为指标，在单因素试验基础上，通过响应面分析法考察浸提时间、乙醇体积分数及pH对桑叶中1-DNJ提取工艺的影响，利用Design Expert 7.1.6分析软件对试验数据进行分析。结果：最佳工艺参数为乙醇体积分数66.53%，pH 6.66，浸提时间2.73 h；1-DNJ实际提取率0.194 6%，与理论量最优值0.1%相差较小。结论：建立的二次多项数学模型准确可靠，优选的提取工艺稳定可行，为桑叶资源的开发利用提供参考。

Abstract

Objective: To optimize dilute acid ethanol extraction technology of 1-deoxynojirimycin(1-DNJ) from Mori Folium. Method: HPLC-ELSD was adopted to determine the content of 1-DNJ with mobile phase of acetonitrile-water (90:10) and ELSD drift tube temperature of 40 ℃

flow rate of carrier gas was 3.5×105 Pa.With yield of 1-DNJ as index

based on single factors

response surface methodology was adopted to investigated effects of soaking time

ethanol concentration and pH on extraction technology of 1-DNJ

then test data were analyzed by Design Expert 7.1.6 software. Result: Optimum process conditions of 1-DNJ were as follows:ethanol concentration 66.53%

pH value of 6.66

soaking time 2.73 h;Under these conditions

yield of 1-DNJ was 0.194 6%

which had small difference between theoretical optimal value of 0.100%. Conclusion: This established quadratic multinomial model was accurate and reliable

optimized technology could provide a reference for development and utilization of Mori Folium resources.

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