Objective: To prepare W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion. Method: W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion was prepared by two-step method

with centrifugation retention rates as comprehensive evaluation index

orthogonal test was used to investigate effects of dosages of emulsifier Ⅰ and Ⅱ

the concentration of gelatin solution and reaction temperature on formulation technology

then combined with particle morphology and particle size distribution to screen optimum formulation and its characterization. Result: Optimum prescription process was as follows:emulsifier Ⅰ and Ⅱ amounts all of 8%

gelatin solution concentration of 0.25%

reaction temperature at 70 ℃;Under these conditions

parcle rate of total saponins from Ginseng Radix Et Rhizoma was 71.8% with surface tension of 32.2 mN·m-1 and conductivity of 453 μs·cm-1. Conclusion: These W/O/W total saponins from Ginseng Radix Et Rhizoma multiple emulsion was stable

it could provide a reference for improving oral bioavailability of total saponins from Ginseng Radix Et Rhizoma.