Objective: To investigate effects of different penetration enhancers on in vitro transdermal absorption ability of infantile diarrhea gel ointments. Method: Drug transdermal instrument was adopted to investigate percutaneous penetration of infantile diarrhea gel ointments

acceptable solution was selected by single factor test

and then 24 h in vitro total cumulative permeation and cumulative transmittance of evodiamine and retaecarpine in infantile diarrhea gel ointments were investigated after adding different content(1%

3%

5%) azone

oleic acid

propylene glycol.HPLC was adopted to determine contents of evodiamine and retaecarpine by taking acetonitrile-water(51:49) as mobile phase and detection wavelength of 225 nm. Result: Within 24 h

cumulative transmittance of evodiamine and retaecarpine in blank sample was 34.53%

in samples containing different amounts of azone were 33.92%

34.78% and 34.65%

in samples containing different contents of oleic acid were 33.67%

34.57% and 34.57%

in samples containing different contents of propylene glycol were 34.49%

34.61% and 34.53%

respectively. Conclusion: Volatile components from infantile diarrhea gel ointments had promotion role of drug penetration.