Objective: To develop HPLC for the simultaneous determination of four flavonoids in Callicarpa nudiflora. Method: The separation was performed on a Venusil XBP-C18column(4.6 mm×200 mm

5 μm)

the analytes were separated efficiently using the mobile phase consisted of MeOH-H2O in a gradient program. The flow rate was maintained at 1.0 mL·min﹣1.The detective wavelength was set at 350 nm and the column temperature was 25 ℃. Result: There were good linear relationships between the concentrations and the peak area of luteolin-7-O-β-D-glucopyranoside

luteolin

5

4'-dihydroxy-3

7

3'-trimethoxyflavone and 5-hydroxy-3

7

3'

4'-tetramethoxyflavone in the ranges of 0.000 8-0.016 g·L-1(r=0.999 1)

0.001-0.032 g·L-1(r=0.999 1)

0.004-0.08 g·L-1(r=0.999 2)

0.002 4-0.048 g·L-1 (r=0.999 1)

respectively. The recoveries were found to be 98.5% with RSD of 1.7%(n=9)

98.6% with RSD of 1.6%(n=9)

98.6% with RSD of 1.4%(n=9) and 98.6% with RSD of 1.7%(n=9)

respectively. Conclusion: The method is accurate and reproducible for determination of luteolin-7-O-β-D-glucopyranoside

luteolin

5

4'-dihydroxy-3

7

3'-trimethoxyflavone and 5-hydroxy-3

7

3'

4'-tetramethoxyflavone in C. nudiflora.