Objective: A method for simultaneous determination of five ingredients in Wuzi Yanzong pill was developed by reversed-phase high-performance liquid chromatography (HPLC). Method: HPLC was performed on Angilent Extend C18 column (4.6 mm×250 mm

5 μm) with a gradient elution system using methanol:ethylactate(10:1)-0.4% phosphoric acid at 30 ℃ and at a flow rate of 1.0 mL·min-1. The eluate was detected by wavelength of 250 nm for schisandrin

deoxyschizandrin

γ-schizandrin and 360 nm for hyperoside and kaempferide. Result: Hyperoside

kaempferide

schisandrin

deoxyschizandrin and γ-schizandrin were baseline separated and their linear ranges were 4.824-482.4

4.358-87.16

1.854-185.4

5.610-112.2

3.850-77.00 ng

respectively. The recovery of the five compounds (n=9) were 101.2%

98.9%

101.6%

101.3%

97.6%

respectively. Conclusion: The developed method is simple

accurate

and shows good precision and reproducibility. It is could be used for the quality control of Wuzi Yanzong pill.