Objective: To develop an UPLC method of determining the characteristic chromatographic profile of Chrysanthemum indicum for controlling the drug quality quickly and accurately. Method: The analysis was performed on an Agilent C18 column (2.1 mm×50 mm

1.8 μm) with acetonitrile-0.1% phosphoric acid as mobile phase in gradient elution mode.The flow rate was 0.4 mL·min-1 and the injection volume was 1.0 μL with the detection wavelength of 326 nm.The column temperature was set at 25 ℃. Result: The fingerprint of C. indicum was obtained within 12 min

and five peaks in the fingerprint were designed. 15samples were analyzed

and the similarities were 0.871-0.985. Conclusion: The UPLC system enjoys the merits of a higher speed

sensitivity

and resolution

compared with the HPLC system. It can be used to control the quality of Chrysanthemum indicum.