Objective: Using metabonomics method to study the influence of different compatibility proportion of Gastrodia elata and Ligusticum chuanxiong to endogenous metabolites in migraine rats

the purpose of these works was to find potential biomarkers and to analyze the metabolic pathways which can provide new ideas for the pharmacological mechanism of traditional Chinese medicine. Method: The herbs were divided into four groups:G. elata Group

L. chuanxiong group

G. elata and L. chuanxiong A(1:4)

G. elata and L. chuanxiong B (4:1). Each group of herbs was extracted with 70% ethanol (10 times the amount of volume). The rat model of migraine was established by injecting nitroglycerin into the organization of rat. The methods of PCA and PLS-DA were used to analysis the influence of different compatibility proportion of G. elata and L. chuanxiong on endogenous metabolites in migraine rat

and search for biomarkers with load map information. Result: The analysis result of metabolomics showed that:compared with G. elata and L. chuanxiong B group

the score points of G. elata and L. chuanxiong A group were more closer to the normal control group

farer from the model group;16 potential biomarkers were selected from the load plot

and 9 potential biomarkers were identified their structure and analyzed metabolic pathways. Conclusion: Metabolomics can provide detection indexes that reflect the metabolic state of the body. G. elata and L. chuanxiong A group can return the endogenous metabolic phenotype of migraine model rat to normal status. Its mechanism may be involved in vivo physiological activity

such as amino acid metabolism and the citric acid cycle.