Objective: To compare antioxidative activity of Ginkgo biloba fermentation liquid ethyl acetate extract under different fermentation time. Method: The Eurotium cristatum was inoculated into liquid medium

incubation time were 4

7

11

14 d respectively. The ethyl acetate extract of G. biloba aqueous extract was treated as control. E. cristatum and some residual impurities were filtrated to harvest clear fermentation liquor

and extracted by solvent extractions. Antioxidation of ethyl acetate extract was evaluated by 1

1-diphenyl-2-picrylhydrazyl(DPPH)

2

2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS) and ferric reducing antioxidant power (FRAP) assays. The different activitiesfrom fermentation time were compared. Result: All above extracts showed different antioxidant activities

moreover

11 d extracts displayed the best activity:DPPH 50% inhibiting concentration(IC50) 105.7 mg·L-1;ABTS IC50 83.6 mg·L-1

which was close to vitamine C(VC) and the results of absorbance in FRAP test were quite consistent. Conclusion: Antioxidative composition accumulated in G.biloba fermentation liquor was largest at 11 d fermentation time.