Objective: To evaluate the influence of Veratrum nigrum L. to Panax gingseng's anti-fatigue effect with the various physiological and pathological conditions. Method: ICR mice were device two groups

the normal and Qi deficiency groups. The Qi deficiency mice were constructed by administrating of Rhei Radix et Rhizoma and on diet. The uniform design method was used by two factors and seven/eleven levels to investigate the hypothermal swim time in the normal and Qi deficiency mice. Result: For normal mice

dose range of P. gingseng's anti-fatigue effect was 1.00-8.00 g·kg-1. When P. gingseng:V. nigrum<100:1

the total dose was 1.00-9.00 g·kg-1

V. nigrum had the antagonism effect to P. gingseng and the influence would enhance with the increasing of dose. But V. nigrum only decreased the potency not the efficacy of P. gingseng. For Qi deficiency mice

dose range of P. gingseng's anti-fatigue effect was 0.50-8.00 g·kg-1. When P. gingseng:V. Nigrum<100:1

the total dose was 0-9.00 g·kg-1

there was a positive correlation between the hypothermal swim time and the dose of P. gingseng. V. nigrum could not affect the anti-fatigue function of P. gingseng. Conclusion: The mice with the various physiological and pathological conditions

dose of P. gingseng's anti-fatigue effect were different

and the influence of V. nigrum to P. gingseng's anti-fatigue effect was different either.