Objective: To study the intervention effects of Mailuoning injection on the nf-kappa B(NF-κB) and tissue factor(TF) pathway of human umbilical vein endothelial cells (HUVECs) induced by lipopolysaccharide (LPS). Method: HUVECs were cultured in a low-glycemic DMEM containing 10% fetal bovine serum

0.5% penicillin and streptomycin and 10 μg·L-1 basic fibroblast growth factor(bFGF) at 37 ℃. The inflammation cell model of HUVECs was induced by 5 mg·L-1 LPS treatment for 24 hours. The activity of the inflammatory cell model after the treatment of Mailuoning injection was observed by MTT

so as to determine the low

middle

high doses group on Mailuoning injection. And the activities of interleukin-6(IL-6) and tumor necrosis factor alpha (TNF-α) were detected by ELISA. Besides

using Western blotting for detection of NF-κB and TF content. Result: Compared with the model group

effect of Mailuoning in treatment group increased significantly (P<0.05 or P<0.01). But the effect was stable or falling at the dose of 12 mg·L-1

so the low

middle and high dose group of Mailuoning was determined as 4

8

12 mg·L-1

respectively.The level of IL-6 and TNF-α and the NF-κB and TF protein expression of the inflammatory cell model were significantly reduced by each dose group of Mailuoning(P<0.01). Conclusion: Mailuoning injection has a certain relationship between the anti-inflammatory effect and its antagonist of NF-κB and TF.