Objective: To study Liuwei Dihuang wan drug serum on SD rat bone marrow-derived dendritic cells (BMDC) proliferation. Method: Made Liuwei Dihuang wan drug serum

drug serum was observed under the action of cell growth

cell count and cell viability test.Using tetrazolium salt (MTT) method to detect different concentrations and time Liuwei Dihuang wan pills drug serum changes under the action of cell proliferation

finding the best Liuwei Dihuang wan concentration and time. ELISA assay supernatants of dendritic cells secrete cytokines IL-12 concentration. mixed lymphocyte reaction (MLR) test which stimulate T cell activation and proliferation. Result: Compared with the control group

mature DC-containing serum significantly promote the DC cell proliferation

and this proliferation of drug-containing concentrations of 15%

reaction time 48h of the most significant. Its IL-12 secretion significantly increased (P<0.05). Liuwei Dihuang wan drug serum mature DC cultured allogeneic lymphocytes proliferation stimulating effect

and with the enhanced role of the increase in the number of cells (P<0.05)

showed a dose-effect relationship. Conclusion: Liuwei Dihuang wan drug serum has induced the role of rat bone marrow dendritic cells proliferation.