Objective: To explore the main ingredients from Motherwort injection for coagulation system. Method: By measuring the coagulation values

platelet aggregation and fibrinolysis to explore the effects of the main ingredients from Motherwort injection on blood coagulation system. Result: In vitro

trigonelline obviously prolonged activated partial thromboplastin time(APTT)

both choline chloride and trigonelline inhibited platelet aggregation

but no significant fibrinolytic activity. Conclusion: Both choline chloride and trigonelline are the active ingredients of Motherwort injection for coagulation system. The extraction and separation of active ingredients expand the clinical application of Motherwort injection and provide a good scientific basis for the quality control of Motherwort injection.