胡芦巴总皂苷的提取纯化工艺考察

黑晶; 陈挚; 雷亚亚; 王文苹; 隋宏

doi:10.11653/syfj2014050011

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胡芦巴总皂苷的提取纯化工艺考察

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- 胡芦巴总皂苷的提取纯化工艺考察
- Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen
- 中国实验方剂学杂志 2014年20卷第5期页码：11-13
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81160548）;宁夏科技攻关项目（宁科计字[2010]170号）
- DOI：10.11653/syfj2014050011
  中图分类号：
- 纸质出版日期：2014
- 稿件说明：
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黑晶, 陈挚, 雷亚亚, 等. 胡芦巴总皂苷的提取纯化工艺考察[J]. 中国实验方剂学杂志, 2014,20(5):11-13.

HEI Jing, CHEN Zhi, LEI Ya-ya, et al. Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 11-13.
黑晶, 陈挚, 雷亚亚, 等. 胡芦巴总皂苷的提取纯化工艺考察[J]. 中国实验方剂学杂志, 2014,20(5):11-13. DOI： 10.11653/syfj2014050011.

HEI Jing, CHEN Zhi, LEI Ya-ya, et al. Investigation of Extraction and Purification Process for Total Saponins in Trigonellae Semen[J]. Chinese journal of experimental traditional medical formulae, 2014, 20(5): 11-13. DOI： 10.11653/syfj2014050011.

摘要

目的：制备高纯度的胡芦巴总皂苷。方法：采用乙醇回流法提取胡芦巴总皂苷，依次加正己烷、三氯甲烷、乙酸乙酯、正丁醇进行系统溶剂萃取，三氯甲烷有效部位经HPD-400型大孔吸附树脂富集纯化。采用显色反应和TLC鉴别总皂苷，运用UV测定胡芦巴总皂苷含量。结果：三氯甲烷萃取部位中总皂苷纯度40%，得率6.28%；水洗脱液中胡芦巴总皂苷纯度80.5%，得率0.32%。结论：优选的提取纯化工艺快速简便，为胡芦巴总皂苷的药效学评价奠定基础。

Abstract

Objective: To prepare total saponins of Trigonellae Semen with high purity. Method: Total saponins of Trigonellae Semen was extracted by ethanol refluxing method

then extract was dealt with different solvents systematically

including n-hexane

chloroform

ethyl acetate

n-buthanol.Chloroform effective part was purified through HPD-400 macroreticular resin.Total saponins in each steps and fractions was identified by chromogenic reaction and TLC

contents of total saponins were determined by ultraviolet spectroscopy. Result: Purity of total saponins in chloroform solvent extraction was 40% with yield of 6.28%;Purity of total saponins in water eluent was 80.5% with yield of 0.32%. Conclusion: This optimized process was convenient and efficient

it could lay foundation for pharmacodynamic study.

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