Objective: To investigate effects of ultrafiltration process on transmittance of active ingredients from Shengmai injection. Method: With transmittance of ginsenosides Rg1

Re

Rb1 and schisandrin as indexes

orthogonal test was adopted to investigate effects of temperature

pressure and polysulfone membrane aperture on ultrafiltration process of Shengmai injection.HPLC was adopted to determine contents of index components with mobile phase of acetonitrile(A)-water(B)(0-30 min

0%-10% A;30-40 min

10%-23% A;40-50 min

23% A;50-85 min

23%-60% A;85-95 min

60%-100% A) and detection wavelength at 203 nm. Result: The best ultrafiltration process was as follows:temperature45 ℃

pressure 11 psi

polysulfone membrane aperture 50K;Under these conditions

transmittance of ginsenosides Rg1

Re

Rb1 and schisandrin were 80.26%

85.02%

50.69%

94.99%

respectively. Conclusion: Ultrafiltration process had a certain effect on transmittance of active ingredients from Shengmai injection

it could provide experimental basis for improvement of filtration process of Shengmai injection.