Objective: To establish a high performance liquid chromatographic method (HPLC) for simultaneous determination of rubrofusarin gentiobioside

casside

cassiaside C in Cassia obtusifolia. Method: The HPLC with Dionex Acclaim C18(4.6 mm×250 mm

5 μm) column was used

acetonitrile-THF-1% acetic acid (17 ：2 ：81) was used as a mobile phase

with flow rate of 1 mL·min-1

column temperature at 30 ℃ and detection wavelength at 278 nm. Result: Rubrofusarin gentiobioside

casside

cassiaside C showed good linearity (r=0.999 9) in the range of 0.218-1.09

0.188-0.94

0.200-1.000 μg

regressive equation were Y=54.017X-0.344 6 (r=0.999 9)

Y=78.63X-0.354(r=0.999 9)

Y=74.098X-0.433 5 (r=0.999 9)respectively;the average recoveries of the method were 98.43%

101.89%

102.46%

RSD were 1.75%

1.10%

1.15%. Conclusion: The method was proved to be simple

rapid

sensitive

precise

reliable and repeatable. It can be applied to the quality control of Semen Cassia.